Figure 11.

Localization of mid1p and NLS*-mid1p in plo1–1 mutant. Exponential cultures of plo1–1 mutant (A; YDM 109 strain) and plo1–1 mutant expressing NLS*-mid1 as the only mid1 protein (B; AP370 strain) were grown at 25°C, shifted for 2 h at 36°C, and fixed and stained with anti-mid1p Igs (top) and DAPI (bottom). mid1p is present in the nucleus and in a central broad band in interphase (left), mitotic (middle), and post mitotic (right) cells. NLS*-mid1p forms a broad cortical band in interphase and mitosis. Bars : 2.5 μm.