Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Complementation of mid1 deletion by mutated mid1 constructs. Strains SP1601 (A), AP250 (B), AP82 (C), AP251 (D), AP80 (E), AP76 (F), and AP85 (G) were grown at 30°C (left) or 36°C (right) and stained for cell wall and septum with calcofluor (see Table 2 for statistics). Bar: 10 μm. Diagrams of mid1 mutant constructs are shown on the right. Bottom: Western blot analysis of expression levels of mid1 mutant constructs at 30 and 36°C. wt: wild-type strain FC 418. A-G: see above.
