FIG. 3.
Representative examples of polykaryocytes in 143TK− cells (A to D) or BHK cells (E to H) induced in BHK cells. 143TK− cells were cotransfected with plasmids encoding gB, gH/gL, and a mutant gD or wild-type gD, together with a plasmid encoding lacZ. Transfected cells were stained with X-Gal. (A) Wild-type gD. (B) ▿77a. (C) ▿77b. (D) gD alone. BHK cells were cotransfected with plasmids encoding gB, gH/gL, and a mutant gD or wild-type gD, together with a plasmid encoding lacZ. Transfected cells were stained with X-Gal at 40 h after transfection. (E) Wild-type gD. (F) ▿77a. (G) ▿77b. (H) Background resulting from transfection of wild-type gD in the absence of other glycoproteins.