FIG. 2.
(A) p24gag antigen accumulation in the culture medium of HIV-1NL4-3-infected NHOKs. The cell-free culture medium of HIV-infected NHOKs was removed at the times indicated for measurement of p24gag levels by ELISA. The graph shows p24 levels versus the number of days postinfection, and the results are averages of three independent experiments. HI, heat-inactivated virus. (B) Experiment similar to that in panel A, except that the replication of HIV-1NL4-3 was compared between NHOKs and PBLs by assaying p24 levels by the ELISA method. (C) Summary of p24 levels in T-lymphotropic NL4-3-, ELI-, dualtropic 89.6-, and CCR5-tropic Ba-L-infected NHOKs.