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HIV-1NL4-3 requires GalCer and/or CXCR4 for NHOK infection. NHOKs were pretreated with various HIV-1 entry inhibitors for 1 h at 37°C and then exposed to HIV-1NL4-3 for 1 h at 37°C. The final concentrations of the inhibitors were 10 μg of recombinant soluble CD4 per ml, 100 ng of SDF-1α per ml, 50 μg of nonspecific MAb per ml, and 2.5 μg of anti-GalCer MAb per ml. Viral replication was analyzed by determining the p24 antigen concentration in the postinfection culture supernatant.
