FIG. 5.
(A) Viral transmission from NHOKs to PBLs during coculture. NHOKs (5 × 103) were incubated for 2 h in culture supernatants containing approximately 300 ng of HIV-1NL4-3 per ml. Following incubation, NHOKs were washed extensively and cultured overnight with an equal number of uninfected activated PBLs. Nonadherent PBLs were then harvested, washed extensively, and incubated. After 48 h of culture, HIV-1 infection of cocultured PBLs was assessed by PCR amplification of viral early reverse transcripts. HI, heat inactivated. (B) HIV-1NL4-3 replication in coculture assays. p24 levels were assayed by ELISA in supernatants from the final 48 h of PBL culture in the experiment shown in panel A. Triangles, p24 levels of originally infected NHOKs before coculture; squares, p24 levels of rescuing PBLs after overnight incubation with infected NHOKs. (C) Experiment parallel to those in panels A and B following exposure of NHOKs to 300 ng of HIV-1Ba-L per ml for 2 h.