TABLE 3.
Results of PCR-based distinction between M. tuberculosis and M. bovis
| PCR product | Resultb for:
|
|||||
|---|---|---|---|---|---|---|
| M. bovis | mtp40-1/2 | mtp40-3/4 | oxyR | IS6110 | Spoligotyping | |
| Modern control DNA | ||||||
| M. tuberculosis H37Rv (10 ng/μl) | + | ++ | ++ | + | + | +(M. tuberculosis) |
| M. bovis BCG (10 ng/μl) | ++ | + | ++ | + | + | +(M. bovis) |
| M. tuberculosis H37Rv (0.01 ng/μl) | − | + | + | + | + | +(M. tuberculosis) |
| M. bovis BCG (0.01 ng/μl) | + | − | + | + | + | +(M. bovis) |
| Recent autopsy cases | ||||||
| Paraffin sample M. bovis | + | − | − | − | + | +(M. bovis) |
| Paraffin sample M. tuberculosis | − | − | − | − | + | +(M. tuberculosis) |
| Historic tissue samplesa | ||||||
| TT453-9 | + | − | − | − | + | +(M. tuberculosis) |
| TT453-14 | + | − | − | − | + | +(M. tuberculosis) |
| TT95-122 | + | − | − | + | + | +(M. tuberculosis) |
| TT95-40 | − | − | − | + | + | +(M. tuberculosis) |
| TT95-169 | − | − | − | − | + | +(M. tuberculosis) |
| DAN93.11 | + | + | − | − | + | +(M. tuberculosis) |
| DAN95.1-1 | + | − | + | + | + | +(M. tuberculosis) |
| TT196-M5 | − | − | − | − | + | +(M. africanum) |
a
Indicated are the number of the tomb and of the respective specimen.
b
−, no amplification product; +, amplification product; ++, abundant amplification product.