Figure 2.

Analysis of adherens junction components in MDCK cells. Cell lysates were prepared from MDCK parental cells and two representative clones of vector control and PECAM-1 isoform transfected cells under normal growth conditions. Equal amounts of protein (30 μg) were analyzed by SDS-PAGE and Western blotting with specific antibodies to E-cadherin, α-catenin, β-catenin, and γ-catenin. Note the dramatic decrease in production of E-cadherin, α-catenin, and β-catenin but not γ-catenin in Δ15 PECAM-1–expressing cells. These experiments were repeated three times with identical results.