Figure 5.

Inhibition of MAPK/ERKs in Δ15 PECAM-1–expressing cells restores the closely packed epithelial morphology. MDCK cells expressing the Δ15 PECAM-1 isoform were incubated with the vehicle (A), wortmannin (B), or PD98059 (C) in growth medium. The morphology of the cells was monitored microscopically and photographed (10× objective). Note that the closely packed epithelial morphology was observed only when the cells were incubated with PD98059. These experiments were repeated twice with two different batches of the same inhibitors with identical results.