Figure 6.

Transient transfection of MDCK cells with the Δ15 PECAM-1 isoform results in activation of MAPK/ERKs. MDCK cells were transfected with empty vector, Δ15 PECAM-1, or Δ14&15 PECAM-1, and 48 h later the cells were lysed and equal amounts of protein (30 μg) were analyzed by SDS-PAGE and Western blotting with either antiphospho-MAPK/ERKs (upper panels) or anti-ERK-1 (lower panels). Note the increased levels of phosphorylated active MAPK/ERKs in the Δ15 PECAM-1 transfected cells. These experiments were repeated three times with two different plasmid DNA preparations with identical results.