TABLE 1.
Detection of PRRSV from serum and tonsil tissue samples
| Day p.i. | Virus isolationa
|
RT-PCRb
|
|||
|---|---|---|---|---|---|
| Serum | Tonsil biopsy sample | Serum | Tonsil VI inoculumc | Tonsil biopsy sample | |
| 7 | 28/28 | 26/28 | 27/28 | 27/28 | NTd |
| 14 | 20/28 | 23/28 | 28/28 | 28/28 | NT |
| 28 | 2/28 | 9/28 | 7/28 | 27/28 | NT |
| 56 | 0/28 | 4/28 | 1/28 | 21/28 | NT |
| 84 | 0/28 | 0/28 | 0/28 | 20/28 | NT |
| 119 | NT | 0/28 | 0/28 | 1/28 | NT |
| 147 | NT | 0/28 | 0/28 | 2/28 | 0/28 |
| 168 | NT | 0/28 | 0/28 | 0/28 | 1/28 |
| 196 | NT | 0/28 | 0/28 | 0/28 | 1/28 |
| 225 | NT | 0/28 | 1/28 | 1/28 | 1/28 |
| 251e | NT | 0/28 | 1/28 | 2/28 | 0/28 |
a
Values are numbers of serum or tonsil biopsy samples from which PRRSV was isolated relative to numbers tested.
b
Values are numbers of serum or tonsil samples positive for PRRSV RNA relative to numbers tested by RT-PCR.
c
Tonsil VI inoculum, aliquot of tonsil preparation used for virus isolation.
d
NT, not tested.
e
Samples collected on day 251 p.i. were at necropsy.