TABLE 1.

Immunoreactivity of an antigen capture ELISA to crude preparations of fungal antigens

Antigena	OD at 450 nmb
P. marneffei MCFAg	1.540
P. marneffei YEAg	1.417
C. albicans	0.125
C. neoformans	0.151
H. capsulatum	0.154
A. fumigatus	0.163
A. flavus	0.194
Culture medium (SDA)	0.110

^aFungal strains were obtained from the American Type Culture Collection, Rockville, Md.; the Microbiology Reference Center, Thailand Institute of Science and Technological Research, Bangkok; and Department of Clinical Pathology, Ramathibodi Hospital Faculty of Medicine, Mahidol University. The protein content of each fungal antigen preparation was adjusted to 1 μg/ml. A 50-μl volume was used for analysis. SDA, Sabourand dextrose agar.

^bMean OD determined by duplicate assays performed on three separate occasions.