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Linearity and limits of detection of the qPCR assay. The linear response of the qPCR mecA assay as a function of input genomic DNA (2 fg to 10 ng) purified from strain ATCC33591 is shown. Correlation coefficient, >0.99; slope, −3.59. Values are means ± standard errors of the means of 4 to 16 determinations. ∗, value excluded from linear regression. ∗∗, amounts of input DNA leading to irregular signal detection.
