TABLE 1.

List and characteristics of oligonucleotides used in the triplex qPCR assay

Gene and primer or probe namea	Sequence (5′→3′)	Length	5′ dyeb	GenBank accession no. (reference)
mecA				X52593 (44)
F mecA	CATTGATCGCAACGTTCAATTT	22
R mecA	TGGTCTTTCTGCATTCCTGGA	21
P mecA	TGGAAGTTAGATTGGGATCATAGCGTCAT	29	JOE
femA-SA				X17688 (3)
F femA-SA	TGCCTTTACAGATAGCATGCCA	22
R femA-SA	AGTAAGTAAGCAAGCTGCAATGACC	25
P femA-SA	TCATTTCACGCAAACTGTTGGCCACTATG	29	FAM
femA-SE				U23713 (2)
F femA-SE	CAACTCGATGCAAATCAGCAA	21
R femA-SE	GAACCGCATAGCTCCCTGC	19
P femA-SE	TACTACGCTGGTGGAACTTCAAATCGTTATCG	32	TET

^afemA-SA, S. aureus femA; femA-SE, S. epidermidis femA.

^bAll probes are quenched by TAMRA bound to the 3′ end.