FIG. 5.

Using the same weakly positive patient sample that was used for Fig. 4, hMPV RNA was amplified by single-round RT-PCR using asymmetric primers with a fivefold excess of the sense primer, MPV01.2 (broken line), or of the antisense primer, MPV02.2 (solid line). The kinetic curves indicate that the latter primer mix is superior (A) and this is confirmed by the melt analysis which shows incomplete oligoprobe hydrolysis when using more of the sense primer (B).