Table 2.
2D motility and dynamic morphology parameters of wild-type (Ax4), regA−, and regA−-rescued cells in a spatial gradient of cAMP
| Cell type | Number of cells | Instantaneous velocity (μm/min) | Positive flow (%/min) | Directional change (deg/min) | Maximum length (μm) | Maximum width (μm) | |
|---|---|---|---|---|---|---|---|
| Wild type (Ax4) | 33 | 10.6 (±4.1) | 8.5 (±2.5) | 21.6 (±11.1) | 21.5 (±3.4) | 8.5 (±1.2) | |
| regA− | 29 | 12.1 (±5.2) | 8.8 (±2.9) | 26.8 (±15.5) | 23.7 (±5.3) | 9.0 (±1.1) | |
| regA−-rescued | 20 | 10.1 (±4.7) | 7.2 (±2.0) | 21.7 (±9.2) | 23.4 (±5.2) | 9.2 (±2.0) | |
| Cell type | Number of cells | Area (μm2) | Roundness (%) | Mean convexity (degree) | Mean concavity (degree) | Chemotactic index | Positive chemotaxis (%) |
| Wild type (Ax4) | 33 | 119 (±17) | 51.4 (±8.8) | 574 (±69) | 215 (±70) | +0.61 (±.34) | 90 |
| regA− | 29 | 127 (±36) | 56.0 (±11.4) | 583 (±84) | 228 (±86) | +0.48 (±0.28) | 93 |
| regA−-rescued | 20 | 135 (±43) | 51.1 (±11.0) | 610 (±95) | 250 (±95) | +0.67 (±0.34) | 85 |
Parameters were computed over a 5–10-min period for each cell. In all cases, the mean (± SD) value of each parameter is for the number of cells noted. All measured parameters but the chemotactic index proved statistically indistinguishable among the three cell types, using the Student's t test or a one-tailed test of the null hypothesis, when the distributions were non-Gaussian. All p values for the former parameters were above 0.05. The p values for the chemotactic index between regA− and either wild-type (Ax4) or regA−-rescued cells was <0.05.