TABLE 1.

Linearity and LOD analysis obtained using dilutions of the VQA stock HIV-1 RNA

VQA dilution panel			NucliSens HIV-1 QTa					NASBA HIV-1 QTb
Panel member	No. of HIV-1 copies/mlc	Log HIV-1 copies/ml	No. of samples tested	No. of samples positive	% Positive results	Mean no. of copies/ml	Mean log copies/ml	No. of samples tested	Mean no. of copies/mld	Mean log copies/ml
1	5,400,000	6.73	6	6	100	6,550,000	6.79	0	Not tested
2	2,700,000	6.43	6	6	100	2,800,000	6.44	1	1,600,000	6.20
3	1,350,000	6.13	6	6	100	1,300,000	6.11	1	650,000	5.81
4	675,000	5.83	6	6	100	595,000	5.77	1	390,000	5.59
5	337,000	5.53	6	6	100	275,000	5.44	1	210,000	5.32
6	168,750	5.23	6	6	100	128,000	5.10	1	96,000	4.98
7	84,375	4.93	6	6	100	64,667	4.80	1	56,000	4.75
8	42,187	4.63	6	6	100	29,833	4.47	1	28,000	4.45
9	21,093	4.32	6	6	100	17,167	4.23	1	14,000	4.15
10	10,546	4.02	6	6	100	8,533	3.92	1	4,900	3.69
11	5,273	3.72	6	6	100	4,683	3.65	1	2,200	3.34
12	2,636	3.42	6	6	100	2,400	3.38	1	1,200	3.08
13	1,318	3.12	6	6	100	1,033	3.00	1	540	2.73
14	659	2.82	6	6	100	433	2.57	1	820	2.91
15	329	2.52	6	6	100	455	2.51	1	<LDLe
16	164	2.21	6	5	83	97	1.91	1	<LDL
17	82	1.91	6	3	50	42	1.61	1	<LDL
18	41	1.61	6	4	67	90	1.93	1	<LDL
19	20	1.30	6	1	17	31	1.49	1	<LDL

^aNucliSens HIV-1 QT results for each panel member represent the mean of duplicate testing with each of three kit lots.

^bNASBA HIV-1 RNA QT results were obtained by testing each panel member one time.

^cNominal values.

^dObserved values.

^e<LDL, less than the detectable limit (400 copies/ml).