TABLE 3.

HIV-1 RNA Copy numbers reported for clinical specimens diluted to terminal endpoint^a

Specimen	Dilution tested	NucliSens HIV-1 QT (copies/ml)			NASBA HIV-1 RNA QT (copies/ml)
		Lot 1	Lot 2	Lot 3
9040321	Undiluted	470,000 (5.67)	340,000 (5.53)	470,000 (5.67)	360,000 (5.56)
	1:300	2,500 (3.40)	1,200 (3.08)	1,700 (3.23)	2,000 (3.30)
	1:3,000	81 (1.91)	150 (2.18)	41 (1.61)	<LDLb
	1:30,000	<Lower limitc	<Lower limit	<Lower limit	<LDL
9040423	Undiluted	200,000 (5.30)	92,000 (4.96)	170,000 (5.23)	240,000 (5.38)
	1:100	2,100 (3.32)	1,200 (3.08)	1,600 (3.20)	3,900 (3.59)
	1:1,000	270 (2.43)	81 (1.91)	470 (2.67)	<LDL
	1:10,000	<Lower limit	<Lower limit	<Lower limit	<LDL
9040318	Undiluted	73,000 (4.86)	38,000 (4.58)	82,000 (4.91)	84,000 (4.92)
	1:100	910 (2.96)	580 (2.76)	360 (2.56)	1,300 (3.11)
	1:1,000	42 (1.62)	<Lower limit	120 (2.08)	<LDL
	1:10,000	<Lower limit	<Lower limit	<Lower limit	<LDL
9040338	Undiluted	13,000 (4.12)	6,300 (3.80)	6,800 (3.83)	16,000 (4.20)
	1:10	950 (2.98)	790 (2.90)	1,200 (3.08)	1,100 (3.04)
	1:100	35 (1.54)	<Lower limit	<Lower limit	<LDL
	1:1,000	<Lower limit	<Lower limit	<Lower limit	<LDL
9040500	Undiluted	4,400 (3.64)	2,800 (3.45)	7,800 (3.89)	6,600 (3.82)
	1:5	950 (2.98)	760 (2.88)	1,300 (3.12)	1,100 (3.04)
	1:50	200 (2.30)	28 (1.45)	360 (2.56)	<LDL
	1:500	<Lower limit	<Lower limit	<Lower limit	<LDL
9040428	Undiluted	4,000 (3.60)	2,000 (3.30)	3,700 (3.57)	3,700 (3.57)
	1:5	740 (2.87)	190 (2.28)	500 (2.70)	1,200 (3.08)
	1:20	280 (2.45)	130 (2.11)	<Lower limit	<LDL
	1:200	<Lower limit	<Lower limit	<Lower limit	<LDL

^aValues in parentheses are log₁₀ HIV-1 copies/ml.

^b<LDL, result below the detectable limit for NASBA HIV-1 QT (400 copies/ml).

^cBelow lower limit for NucliSens HIV-1 QT (25 copies/ml).