TABLE 4.

NucliSens HIV-1 QT assay precision estimates

HIV-1 RNA stock dilutiona	Nominal mean value (log10 copies/ml)	No. of specimens that tested positive	Observedb mean value (log10 copies/ml)	Overall SDc (log10 copies/ml)
2	6.43	72	6.52	0.14
5	5.53	72	5.54	0.09
8	4.63	72	4.66	0.12
12	3.42	72	3.52	0.18
14	2.82	72	2.93	0.29
15	2.52	72	2.66	0.28
16	2.21	69	2.26	0.36
17	1.91	51	1.98	0.34

^aSerial dilutions were made from a well-characterized HIV-1 RNA stock (titer = 5.4 × 10⁸ HIV-1 RNA copies/ml) as described in Materials and Methods.

^bObserved mean value obtained for each dilution derived from replicate tests per sample.

^cOverall value for all 552 replicate tests, 0.225.