TABLE 2.
Use and performance of different cell cultures for samples detected by culture and/or NASBA
| Cell culture used for EV isolation | Culture-positive samples (n = 39)a
|
No. (%) of culture-negative, NASBA-positive CSF samples (n = 10) inoculated into cell lined | |||
|---|---|---|---|---|---|
| Total no. (%) of positive samples inoculated into cell line | No. (%) EV positive | No. (%) of times first positive cell line | No. (%) of times only positive cell line | ||
| MRC-5b | 39 (100) | 15 (38) | 6 (15) | 3 (8) | 9 (90) |
| RhMKb | 39 (100) | 22 (51) | 14 (36) | 9 (23) | 8 (80) |
| A549b | 25 (64) | 17 (68) | 11 (44) | 6 (15) | 3 (30) |
| BGMc | 30 (77) | 7 (23) | 7 (23) | 2 (7) | 3 (30) |
| RDc | 28 (72) | 9 (32) | 5 (18) | 2 (7) | 3 (30) |
a
Thirty-six EV and 3 parechoviruses were isolated; parechoviruses were isolated in A549 and/or BGM cells.
b
Cell cultures used year-round.
c
Cell cultures used only during peak EV season (June to October).
d
Only one sample was inoculated into all five cell types; two samples each were inoculated into two, three, or four cell types; three samples were inoculated into only one cell type.