TABLE 1.
Primers for PCR deduced from M. genitalium 16S rRNA gene sequence used in initial evaluation
| Primer | Sequence |
|---|---|
| MG16-45F | 5′ TAC ATG CAA GTC GAT CGG AAG TAG C |
| MG16-181R | 5′ ACC CTT GCA GGT CCT TTC AAC TTT A |
| MG16-252R | 5′ CGT CAT TGC CTT GGT AGG CCA |
| MG16-411R | 5′ TTC TTC CCA AAT AAA AGA ACT TTA CAA TCA A |
| MG16-447R | 5′ AAA CTC CAG CCA TTG CCT GCT AG |
| MG16-455R | 5′ GGT ACA GTC AAA CTC CAG CCA TTG |
| MG16-439F | 5′ GAA TGA CTC TAG CAG GCA ATG GCT G |
| MG16-636R | 5′ TCC AAA ACT CCC TAC CAC ACT CTA GAC TG |
| MG16-1117R | 5′ TCC TCC AAT TTA CAT TAG CAG TCT CGT TAA |
| MG16-1204F | 5′ CAA TGG CCA ATA CAA ACA GTA GCC AA |
| MG16-1301R | 5′ CTG ATT CGC GAT TAC TAG TGA TTC CAG |
| MG16-1428R | 5′ ACC GGT GCT ATC CTT GAC ATG CA |
| MG16-1421R | 5′ GTG CTA TCC TTG ACA TGC ACT TCC AA |
a
Primer combinations MG16S-45F-MG16S-447R and MG16S-1204F-MG16S-1301R (underlined primers) were M. genitalium specific and used in the clinical evaluation.