Figure 2.

Primary structure of the Pas1 protein. (A) Nucleotide and predicted amino acid sequences of the pas1⁺ cDNA. The amino acid sequence is shown below the DNA sequence in a single-letter code. Nucleotide numbering starts at the initiating ATG of the predicted pas1⁺ translation product. (B) Scheme of the Pas1 protein. The cyclin box homologous region is shown in black, and two PEST-rich domains are shown in gray. (C) Amino acid homology in the cyclin box and its upstream region among Pas1p, Pcl1p (Hcs26p) (Ogas et al., 1991), Pcl2p (OrfDp) (Frohlich et al., 1991), Pcl5p, Pcl9p (Measday et al., 1997), and Pho80p (Toh-e and Shimauchi, 1986). Amino acids in which Pas1p is identical with the other five cyclins are shown in black.