TABLE 5.
Percent detection of HCV RNA in specimens subjected to various processing conditions in six collection tube types
Specimen and time (h) | % Detected
|
|||||
---|---|---|---|---|---|---|
SST | K2-EDTA | K2-EDTA (PPTc) | Sodium citrate | ACD | Sodium heparin | |
Whole blooda | ||||||
4 | 100 | 100 | 100 | 100 | 100 | 100 |
6 | 100 | 100 | 100 | 100 | 100 | 100 |
24 | 100 | 100 | 100 | 100 | 100 | 100 |
Decanted sampleb | ||||||
0 | 100 | 100 | 98.3 | 100 | 100 | 100 |
8 | 100 | 100 | 100 | 98.3 | 100 | 96.0 |
24 | 100 | 100 | 100 | 94.9 | 97.4 | 100 |
48 | 100 | 100 | 98.3 | 94.9 | 100 | 100 |
Whole blood with 50 HCV RNA copies/ml was allowed to stand at room temperature for the times indicated prior to processing by centrifugation.
Decanted serum and plasma samples with 50 HCV RNA copies/ml were centrifuged within 4 h of collection and stored at 2 to 8°C for the times indicated prior to freezing.
PPT, plasma preparation tube.