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Sensitivity of the TaqMan RT-PCR assay. Tenfold serial dilutions of the RNA extracted from RSV A2 (0.0023 PFU to 230 PFU) (a) and RSV 2B (0.0018 PFU to 180 PFU) (b) were used in the PCR. Primer-probe pairs specific for RSV A (a) and B (b) were employed to detect the corresponding targets. The negative control contained RNase-free water instead of RNA template.
