TABLE 1.

Oligonucleotide primers and probes for NASBA,^a standard RT-PCR, and TaqMan assays

Primer	Coding location	Sequence (5′-3′)	Product size (bp)
EEE NASBA
EEE 9597	9597-9616	gatgcaaggtcgcatatgagCACATGGATGGCCGCACGAA	208
EEE 9804c	9804-9783	aattctaatacgactcactatagggagaaggCAGCAAAGTAACGCCAGGAGTA
EEE 9619probe	9619-9643	GGTAGTCTATTACTACAACAGATAC
EEE RT-PCR
EEE 5640	5640-5659	CGGCAGCGGAATTTGACGAG	433
EEE 6072C	6047-6072	ACTTTGACGGCCACTTCTGCTGATGA
EEE TaqMan
EEE 9391	9391-9411	ACACCGCACCCTGATTTTACA	69
EEE 9459c	9459-9439	CTTCCAAGTGACCTGGTCGTC
EEE 9414probe	9414-9434	TGCACCCGGACCATCCGACCT
WEE NASBA
WEE 9336	9336-9356	gatgcaaggtcgcatatgagCGAGCAGACGCAACAGCAGAA	233
WEE 9566C	9566-9545	aattctaatacgactcactatagggagaaggCAGGATAGCAAGAGCGACACCA
WEE 9390probe	9390-9414	GTGGGGCGAGAAGGGCTGGAGTACG
WEE RT-PCR
WEE 5100	5100-5122	GTTTGGCGGCGTCTCGTTCTCTA	338
WEE 5437c	5437-5414	TCCGTGGTGCTGGTACTGGTCTGT
WEE TaqMan
WEE 10,248	10,248-10,267	CTGAAAGTCGGCCTGCGTAT	67
WEE 10,314c	10,314-10,295	CGCCATTGACGAACGTATCC
WEE 10,271probe	10,271-10,293	ATACGGCAATACCACCGCGCACC

^aNASBA forward primers have 5′ECL sequences (lowercase, italic); reverse primers have T7 promoter sequences (lowercase, italic).