TABLE 3.

Sensitivities and specificities of WEE virus NASBA, standard RT-PCR, TaqMan, and Vero cell culture assays^a

Sample (location and of collection)	Quantity (PFU)	NASBAb		Standard RT-PCR	TaqManc
		ECL units	Int.		CT	Int.
Titrated WEE Fleming seed (California, unknown)
WEE-1	3,500,000	1,387,131	POS	POS	9	POS
WEE-2	350,000	1,309,269	POS	POS	12.1	POS
WEE-3	35,000	1,259,877	POS	POS	15.7	POS
WEE-4	3,500	1,403,760	POS	POS	19.1	POS
WEE-5	350	1,023,508	POS	POS	22.5	POS
WEE-6	35	874,991	POS	POS	26.4	POS
WEE-7	3.5	575	POS	EQUIVd	29.7	POS
WEE-8	0.35	174	NEG	EQUIVd	32.3	POS
WEE-9	0.035	119	NEG	NEG	36.9	POS
WEE-10	0.0035	106	NEG	NEG	45	NEG
WEE virus strains
WEE (Cuba, 1971)	ND	2,046,014	POS	POS	9.2	POS
WEE (Tex., 1973)	ND	2,072,176	POS	POS	9.4	POS
WEE (Argentina, 1982)	ND	1,089,793	POS	POS	29.5	POS
WEE (S.D., 1983)	ND	1,244,439	POS	POS	10.7	POS
WEE (N.Mex., 1984)	ND	2,258,732	POS	POS	14.1	POS
WEE (Tenn., 1987)	ND	1,814,717	POS	POS	16.7	POS
WEE (Canada, 1941)	ND	1,629,668	POS	POS	9.3	POS
WEE (Brazil, 1966)	ND	2,318,310	POS	POS	14.6	POS
WEE (Neb., 1997a)	ND	1,989,971	POS	POS	14	POS
WEE (Neb., 1997b)	ND	2,101,613	POS	POS	15	POS
WEE (Neb., 1997c)	ND	1,960,286	POS	POS	12	POS
WEE (Neb., 1997d)	ND	833,799	POS	POS	21	POS
Other viruses
EEE NJ/60	ND	95	NEG	NEG	45	NEG
Whataroa M78	ND	117	NEG	NEG	45	NEG
Kyzylgach LIEV65A	ND	106	NEG	NEG	45	NEG
Fort Morgan CM4-146(A)	ND	150	NEG	NEG	45	NEG
Highlands J B230	ND	85	NEG	NEG	45	NEG
Buggy Creek 81V1822	ND	86	NEG	NEG	45	NEG
Aura Bear 10135	ND	140	NEG	NEG	45	NEG
VEE TC83	ND	97	NEG	NEG	45	NEG
LaCrosse Original	ND	128	NEG	NEG	45	NEG
Dengue-2 New Guinea	ND	104	NEG	NEG	45	NEG
Powassan LB	ND	35	NEG	NEG	45	NEG
Yellow Fever 17-D	ND	118	NEG	NEG	45	NEG

^aAbbreviations: POS, positive; NEG, negative; EQUIV, equivocal; ND, not determined; Int., interpretation.

^bNASBA-ECL units of >300 are interpreted as positive.

^cTaqMan C_T values of ≤37 are interpreted as positive (Rn values not included).

^dEquivocal standard RT-PCR result determined by the presence of a faint DNA band.