TABLE 4.

Detection of North American EEE virus and viral RNA from field-collected mosquitoes and vertebrate tissues by Vero cell culture, NASBA, standard RT-PCR, and TaqMan assays^a

Sample (location and yr of collection)	Vero cell culture result	NASBAb		Standard RT-PCR	TaqManc		EEE virus infection inferred bye:
		ECL units	Int.		CT	Int.
Field-collected mosquitoes
Mosquito pool 1 (Va., 2001)	POS	107,036	POS	POS	23.2	POS	TaqMan
Mosquito pool 2 (Va., 2001)	POS	83,055	POS	POS	24.1	POS	TaqMan
Mosquito pool 3 (Va., 2001)	POS	32,274	POS	POS	25.3	POS	TaqMan
Mosquito pool 4 (Va., 2001)	POS	127,581	POS	POS	24.2	POS	TaqMan
Mosquito pool 5 (Va., 2001)	NEG	33,117	POS	NEG	34.8	POS	TaqMan
Mosquito pool 6 (N.J., 2000)	NEG	107	NEG	NEG	45	NEG	NT
Mosquito pool 7 (N.J., 2000)	NEG	103	NEG	NEG	45	NEG	NT
Mosquito pool 8 (N.J., 2000)	NEG	175	NEG	NEG	45	NEG	NT
Mosquito pool 9 (N.J., 2000)	NEG	187	NEG	NEG	45	NEG	NT
Mosquito pool 10 (N.J., 2000)	NEG	164	NEG	NEG	45	NEG	NT
Vertebrate tissues
Equine cortex (N.C., 2000)	NEG	79,899	POS	EQUIVd	31.3	POS	Histopathology
Equine cerebellum (N.C., 2000)	NEG	56,367	POS	NEG	37.6	EQUIV	Histopathology
Equine brain stem (N.C., 2000)	NEG	200	NEG	EQUIVd	30.2	POS	Histopathology
Avian heart (Maine, 2001)	NEG	233	NEG	EQUIVd	32.4	POS	Isolation; FA
Human brain (Mich., 2001)	NEG	230	NEG	NEG	32.8	POS	Serology
Avian brain 1 (N.J., 2000)	NEG	113	NEG	NEG	45	NEG	NT
Avian brain 2 (N.J., 2000)	NEG	156	NEG	NEG	45	NEG	NT
Avian brain 3 (N.J., 2000)	NEG	140	NEG	NEG	45	NEG	NT
Avian brain 4 (N.J., 2000)	NEG	137	NEG	NEG	45	NEG	NT
Avian brain 5 (N.J., 2000)	NEG	127	NEG	NEG	45	NEG	NT

^aAbbreviations: POS, positive; NEG, negative; EQUIV, equivocal; NT, not tested.

^bNASBA-ECL units of >300 are interpreted as positive.

^cTaqMan C_T values of ≤37 are interpreted as positive (Rn values not included).

^dEquivocal standard RT-PCR result determined by the presence of a faint DNA band.

^eSample submitted to CDC molecular diagnostic laboratory for confirmation of EEE virus infection inferred by testing at another laboratory.