TABLE 4.
Sample (location and yr of collection) | Vero cell culture result | NASBAb
|
Standard RT-PCR | TaqManc
|
EEE virus infection inferred bye: | ||
---|---|---|---|---|---|---|---|
ECL units | Int. | CT | Int. | ||||
Field-collected mosquitoes | |||||||
Mosquito pool 1 (Va., 2001) | POS | 107,036 | POS | POS | 23.2 | POS | TaqMan |
Mosquito pool 2 (Va., 2001) | POS | 83,055 | POS | POS | 24.1 | POS | TaqMan |
Mosquito pool 3 (Va., 2001) | POS | 32,274 | POS | POS | 25.3 | POS | TaqMan |
Mosquito pool 4 (Va., 2001) | POS | 127,581 | POS | POS | 24.2 | POS | TaqMan |
Mosquito pool 5 (Va., 2001) | NEG | 33,117 | POS | NEG | 34.8 | POS | TaqMan |
Mosquito pool 6 (N.J., 2000) | NEG | 107 | NEG | NEG | 45 | NEG | NT |
Mosquito pool 7 (N.J., 2000) | NEG | 103 | NEG | NEG | 45 | NEG | NT |
Mosquito pool 8 (N.J., 2000) | NEG | 175 | NEG | NEG | 45 | NEG | NT |
Mosquito pool 9 (N.J., 2000) | NEG | 187 | NEG | NEG | 45 | NEG | NT |
Mosquito pool 10 (N.J., 2000) | NEG | 164 | NEG | NEG | 45 | NEG | NT |
Vertebrate tissues | |||||||
Equine cortex (N.C., 2000) | NEG | 79,899 | POS | EQUIVd | 31.3 | POS | Histopathology |
Equine cerebellum (N.C., 2000) | NEG | 56,367 | POS | NEG | 37.6 | EQUIV | Histopathology |
Equine brain stem (N.C., 2000) | NEG | 200 | NEG | EQUIVd | 30.2 | POS | Histopathology |
Avian heart (Maine, 2001) | NEG | 233 | NEG | EQUIVd | 32.4 | POS | Isolation; FA |
Human brain (Mich., 2001) | NEG | 230 | NEG | NEG | 32.8 | POS | Serology |
Avian brain 1 (N.J., 2000) | NEG | 113 | NEG | NEG | 45 | NEG | NT |
Avian brain 2 (N.J., 2000) | NEG | 156 | NEG | NEG | 45 | NEG | NT |
Avian brain 3 (N.J., 2000) | NEG | 140 | NEG | NEG | 45 | NEG | NT |
Avian brain 4 (N.J., 2000) | NEG | 137 | NEG | NEG | 45 | NEG | NT |
Avian brain 5 (N.J., 2000) | NEG | 127 | NEG | NEG | 45 | NEG | NT |
Abbreviations: POS, positive; NEG, negative; EQUIV, equivocal; NT, not tested.
NASBA-ECL units of >300 are interpreted as positive.
TaqMan CT values of ≤37 are interpreted as positive (Rn values not included).
Equivocal standard RT-PCR result determined by the presence of a faint DNA band.
Sample submitted to CDC molecular diagnostic laboratory for confirmation of EEE virus infection inferred by testing at another laboratory.