TABLE 5.
Sample (location and yr of collection) | Vero cell culture result | NASBAb
|
Standard RT-PCR | TaqManc
|
Wee virus infection inferred byd: | ||
---|---|---|---|---|---|---|---|
ECL units | Int. | CT | Int. | ||||
Field-collected mosquitoes | |||||||
Mosquito pool 1 (N.Mex., 2001) | ND | 4,733 | POS | POS | 34 | POS | Standard RT-PCR |
Mosquito pool 2 (N.Mex., 2001) | NEG | 51,943 | POS | POS | 29.7 | POS | Standard RT-PCR |
Mosquito pool 3 (N.Mex., 2001) | NEG | 244 | NEG | POS | 30 | POS | Standard RT-PCR |
Mosquito pool 4 (N.J., 2000) | NEG | 79 | NEG | NEG | 45 | NEG | NT |
Mosquito pool 5 (N.J., 2000) | NEG | 177 | NEG | NEG | 45 | NEG | NT |
Mosquito pool 6 (N.J., 2000) | NEG | 149 | NEG | NEG | 45 | NEG | NT |
Mosquito pool 7 (N.J., 2000) | NEG | 122 | NEG | NEG | 45 | NEG | NT |
Mosquito pool 8 (N.J., 2000) | NEG | 131 | NEG | NEG | 45 | NEG | NT |
Vertebrate tissues | |||||||
Avian brain 1 (N.J., 2000) | NEG | 186 | NEG | NEG | 45 | NEG | NT |
Avian brain 2 (N.J., 2000) | NEG | 121 | NEG | NEG | 45 | NEG | NT |
Avian brain 3 (N.J., 2000) | NEG | 137 | NEG | NEG | 45 | NEG | NT |
Avian brain 4 (N.J., 2000) | NEG | 135 | NEG | NEG | 45 | NEG | NT |
Avian brain 5 (N.J., 2000) | NEG | 87 | NEG | NEG | 45 | NEG | NT |
Abbreviations: POS, positive; NEG, negative; ND, not determined; NT, not tested.
NASBA-ECL units of >300 are interpreted as positive.
TaqMan CT values of ≤37 are interpreted as positive (Rn values not included).
Sample submitted to CDC molecular diagnostic laboratory for confirmation of WEE virus infection inferred by testing at another laboratory.