TABLE 5.

Detection of WEE virus and viral RNA from field-collected mosquitoes and vertebrate tissues by Vero cell culture, NASBA, standard RT-PCR, and TaqMan assays^a

Sample (location and yr of collection)	Vero cell culture result	NASBAb		Standard RT-PCR	TaqManc		Wee virus infection inferred byd:
		ECL units	Int.		CT	Int.
Field-collected mosquitoes
Mosquito pool 1 (N.Mex., 2001)	ND	4,733	POS	POS	34	POS	Standard RT-PCR
Mosquito pool 2 (N.Mex., 2001)	NEG	51,943	POS	POS	29.7	POS	Standard RT-PCR
Mosquito pool 3 (N.Mex., 2001)	NEG	244	NEG	POS	30	POS	Standard RT-PCR
Mosquito pool 4 (N.J., 2000)	NEG	79	NEG	NEG	45	NEG	NT
Mosquito pool 5 (N.J., 2000)	NEG	177	NEG	NEG	45	NEG	NT
Mosquito pool 6 (N.J., 2000)	NEG	149	NEG	NEG	45	NEG	NT
Mosquito pool 7 (N.J., 2000)	NEG	122	NEG	NEG	45	NEG	NT
Mosquito pool 8 (N.J., 2000)	NEG	131	NEG	NEG	45	NEG	NT
Vertebrate tissues
Avian brain 1 (N.J., 2000)	NEG	186	NEG	NEG	45	NEG	NT
Avian brain 2 (N.J., 2000)	NEG	121	NEG	NEG	45	NEG	NT
Avian brain 3 (N.J., 2000)	NEG	137	NEG	NEG	45	NEG	NT
Avian brain 4 (N.J., 2000)	NEG	135	NEG	NEG	45	NEG	NT
Avian brain 5 (N.J., 2000)	NEG	87	NEG	NEG	45	NEG	NT

^aAbbreviations: POS, positive; NEG, negative; ND, not determined; NT, not tested.

^bNASBA-ECL units of >300 are interpreted as positive.

^cTaqMan C_T values of ≤37 are interpreted as positive (Rn values not included).

^dSample submitted to CDC molecular diagnostic laboratory for confirmation of WEE virus infection inferred by testing at another laboratory.