Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Glycerol gradient sedimentation. Membrane proteins from strain DJ1414-A were solubilized in IP buffer containing n-dodecyl β-d-maltoside and then resolved on an 8–30% glycerol gradient. Cultures had been untreated (A) or treated with 10−7 M α-factor for 5 min (B). Fractions were assayed for Ste2-HA (○) and Ste2-GFP (●) and for marker proteins, BSA (4.3S), mouse IgG (7S), and ATCase (11.7S).
