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C-terminal tail of Ste2 is not required for coprecipitation. Cells expressing both Ste2-T326-HA and Ste2–T326-GFP (strain DJ1417-A) were treated with α-factor and processed for immunoprecipitation as in Figure 2. (A) Anti-HA antibody precipitates Ste2-T236-GFP. (B) Anti-GFP antibody precipitates Ste2-T326-HA.
