Table 2.

Quantitation of fluorescent foci from cells expressing Ste2 and Ste2-S184R

Ste2 forms expressedb	α-Factor	Percentage of cellsa
		n ≥ 1	n ≥ 3
Ste2-GFP	−	31  ± 3	7  ± 2
Ste2-GFP	+	94  ± 2	44  ± 3
Ste2-S184R-GFP	−	31  ± 3	6  ± 1
Ste2-S184R-GFP	+	57  ± 3	7  ± 2
Ste2-S184R-GFP and Ste2	−	33  ± 3	7  ± 2
Ste2-S184R-GFP and Ste2	+	72  ± 2	32  ± 2

^a Fluorescent foci were counted from GFP images of cells treated with cycloheximide and α-factor as in Fig. 3. The percentage of cells with one or more fluorescent foci (n ≥ 1) and the percentage of cells with three or more fluorescent foci (n ≥ 3) are indicated for the cells that had been cultured in the presence or absence of α-factor. More than 200 cells were examined for each entry. 

^b The forms of Ste2 expressed in each strain are shown. Strains used are DJ1408-A (rows 1 and 2), DJ1407-A (rows 3 and 4), and DJ1406-A (rows 5 and 6).