Table 4.

Quantitation of fluorescent foci from cells expressing Ste2 and Ste2-T326

Ste2 forms expressedb	α-Factor	Percentage of cellsa
		n ≥ 1	n ≥ 3
Ste2-GFP	−	42  ± 9	9  ± 5
Ste2-GFP	+	100	71  ± 11
Ste2-T326-GFP	−	5  ± 3	5  ± 3
Ste2-T326-GFP	+	5  ± 3	2  ± 2
Ste2-T326-GFP and Ste2	−	10  ± 5	7  ± 5
Ste2-T326-GFP and Ste2	+	74  ± 4	36  ± 5

^a Fluorescent foci were counted from GFP images of cells treated with cycloheximide and α-factor as in Fig. 3. The percentage of cells with one or more fluorescent foci (n ≥ 1) and the percentage of cells with three or more fluorescent foci (n ≥ 3) are shown in the presence or absence of α-factor. 

^b The forms of Ste2 expressed in each strain are shown on the leftmost column. Strains used are DJ1413-A (rows 1 and 2), DJ1410-A (rows 3 and 4), and DJ1411-A (rows 5 and 6).