Figure 10.
TSP1 peptide 678 inhibits wound healing of BAE cells. BAE cells were seeded at a density of 2 × 105 cells/well of six-well tissue culture plates in complete growth medium supplemented with 10% FBS. After the cells formed a confluent cobblestone, cells were arrested with the use of 10 μg/ml 5-fluorouracil for 48 h. Scrape wounds of 2 mm width were made in the wells, and the cells were further incubated with medium containing 10% FBS, 10 μg/ml 5 fluorouracil, and peptides 686 (closed bars) or 690 (striped bars). Measurements of the distance between the wound margins were taken at 0 and 24 h, and the net migrations for a representative experiment of three performed are presented as means ± SEM (n = 3). Significant inhibition relative to the control (p < 0.05) is indicated by an asterisk.