TABLE 1.

ISFtu1 and ISFtu2 RFLPs of F. tularensis isolates

Species and lane no.a	Origin	Strain designation	Alternative strain designation	ISFtu1 RFLP patternb			ISFtu2 RFLP pattern
				PstI	Bg/II	SwaI	PstI	Bg/II	SwaI
F. tularensis subsp.     tularensis
3	Human ulcer, 1941, Ohio	FSC237	Schu-S4	1	I	i	A	a	α
4	Mite, 1998, Slovakia	FSC199	SE-221-38	1	I∗	i	A	a	α
5	Human lymph node, 1920, Utah	ATCC 6223	FSC230, B-38	2	II	ii	B	b	β
6	Tick, 1935, British Columbia, Canada	FSC041	Vavenby	1∗	I∗∗	i	A	a	α
F. tularensis subsp.     holarctica
7	Live vaccine strain, NDBR lot 11	LVS	Live vaccine strain	3	III	iii	C	c	χ
8	Hare, Telemark, Norway, 1963	FSC092	HN63	3∗	IV	iii	D	c	χ
9	Human lymph node, 1926, Japan	FSC017	Jap-S2	4	V	iv	E	d	δ
10	Ticks, 1957, Japan	FSC075	Jama	4	V∗	iv	E	d	δ
11	Beaver, 1976, Montana	FSC035	B423A	3∗	IV	iii	D	c	χ
12	Human, 1998, Ljusdal, Sweden	FSC200		3∗	III	iii	C∗	c	χ
13	Hare, 1952, France	FSC025	Chateauroux	3∗	III∗	iii	D	c	χ∗
14	Human, 1981, Ljusdal, Sweden	FSC108	R 45	3∗	III	iii	C∗	c	χ∗
15	Human Blood, 1996, Raahe, Finland	FSC250	1532	3∗	III	iii	C∗	c	χ
16	Human Ulcer, 1984, Norway	FSC097	83933/84	3∗	III	iii	C∗	c	χ
17	Water, 1990, Odessa region, Ukraine	FSC124	14588	3∗	III	iii	C∗	c	χ
F. tularensis subspecies     mediaasiatica
18	Miday gerbil, 1965, Central Asia	FSC147	543	5	VI	v	F	e	ɛ
19	Tick, 1982, Central Asia, former USSR	FSC148	240	5∗	VI	v	F	e	ɛ

^aLane number in Fig. 2 to 5.

^bIdentical RFLP patterns have each been designated the same arbitrary letter or number code; subtle differences (one or two bands) in an otherwise identical pattern are indicated by asterisks. The restriction enzymes used for digestion of genomic F. tularensis DNA were PstI, BglII, and SwaI.