FIG. 1.
Melting curve analysis of the 267-bp fragment of the 23S rRNA gene obtained with real-time PCR for the wild-type and mutant strains with the A2142C, A2142G, and A2143G mutations. The melting curve corresponding to a biopsy containing the wild type and the A2142C, A2142G, and A2143G mutations is also shown, and three melting peaks can be observed with the Tms corresponding to those of the wild type, the A2142C mutation, and the A224G transition. Values on the y axis represent the ratio of the first negative derivative of the change in fluorescence (dF) to the variation in temperature (temperature values are given in degrees Celsius). A→G, A to G mutation.