TABLE 2.
Results of the PCR-RFLP analysis on 30 biopsies with a single genotype (10 wild type, 19 with the A2142/43G mutation, and 1 with the A2143C mutation) and on all 42 samples with more than one genotype detected by real-time PCR
| Genotype detected by real-time PCR | PCR-RFLP status (no. of samples) |
|---|---|
| Wild type | NR (10)a |
| A→G | BbsI (4), BsaI (15) |
| A→C | BceAI (1) |
| Wild type and A→G | BbsI and NR (15), BsaI and NR (20) |
| Wild type and A→G | BbsI and BsaI and NR (1) |
| Wild type and A→C | BceAI and NR (2) |
| A→G and A→C | BbsI and BceAI (2) |
| A→G and A→C | BsaI and BceAI (1) |
| Wild type and A→G and A→C | BbsI and BsaI and BceAI and NR (1) |
a
NR, not restricted.
b
The melting peaks of this biopsy are represented in Fig. 1.