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Biological activities of Gas3/PMP22 mutated at the N-glycosylation site. gas3/PMP22-MG, gas3/PMP22-WT, and gas3/PMP22-KK were coexpressed with hPLAP in NIH3T3 cells. At different time points after microinjection, cells were fixed and processed by immunofluorescence to detect hPLAP. Survival and morphologic changes were scored as described in the text. The data represent the arithmetic means ± SD of four independent experiments (altered shape, p < 0.001; survival rate, p < 0.001)
