TABLE 1.
Overview of animals, their origin, duration of disease, detection of herpesvirus DNA, and serological results
| Animal | Farm | Date | Duration of disease | Tissue | Viral DNA (copies per 25 mg of tissue) | Conventional PCRa | Serology |
|---|---|---|---|---|---|---|---|
| Pig 1 | B | May 1986 | >5 days | Cerebrum | 144,334 | Pan; OV | NA |
| Cerebellum | 443,233 | ND | |||||
| Kidney | 94,317 | ND | |||||
| Nasal mucosa | 10,961 | ND | |||||
| Pig 2 | A | April 1999 | ∼5 days | Cerebrum | 172,627 | Pan; OV | NA |
| Cerebellum | 29,843 | ND | |||||
| Pig 3 | A | June 2000 | ∼2 days | Cerebrum | 80,918 | Pan; OV | Negative |
| Cerebellum | 103,004 | ND | |||||
| Pharynx | 18,060,515 | ND | |||||
| Skin | 17,268,761 | ND | |||||
| Pig 4 | A | June 2000 | ∼2 days | Cerebrum | 4 | 0 | Negative |
| Cerebellum | 0 | 0 | |||||
| Pig 5 | A | June 2000 | >5 days | Cerebrum | 3,071,006 | Pan; OV | Positive |
| Cerebellum | 683,662 | ND | |||||
| Spinal cord | 622,910 | ND | |||||
| Lung | 633,163 | ND | |||||
| Pig 6 | A, 4339 | Unspec.b | Cerebrum | 0 | Pan PLHV-1/2c | Negative | |
| Pig 7 | H, B-179 | Healthy | Cerebrum | 0 | Pan PLHV-3 | NA | |
| Pig 8 | G, 965-1497 | Healthy | Cerebellum | 0 | Pan | NA |
a
With pan-herpesvirus (pan) or ovine herpesvirus 2 (OV) primers, as shown. ND, not determined; NA, samples not available. Animals presenting only negative results were not included in the table.
b
Unspecific disease signs, not typical for porcine MCF.
c
Sequence determination of the amplification product identified the virus as porcine lymphotropic herpesvirus 1 (PLHV-1).