TABLE 1.
Restriction enzymes, adaptors, and primers
| Restriction enzyme | Recognition site | Adaptor oligonucleotidesa | Primer | Primer name |
|---|---|---|---|---|
| XbaI | 5′T↓CTAGA | 5′CTAGTACTGGCAGACTCT | 5′AGAGTCTGCCAGTACTAGA | px |
| AGATC↓T | 3′ATGACCG | 5′AGAGTCTGCCAGTACTAG | pxn | |
| NheI | 5′G↓CTAGC | 5′AGAGTCTGCCAGTACTAGC | pn | |
| CGATC↓G | 5′AGAGTCTGCCAGTACTAGCG | pn-g | ||
| PstI | 5′CTGCA↓G | 5′CTCGTAGACTGCGTACATGCA | 5′GACTGCGTACATGCAGG | pst-g |
| G↓ACGTC | 3′CATCTGACGCATGT | 5′GACTGCGTACATGCAGAG | pst-ag | |
| 5′GACTGCGTACATGCAGAT | pst-at | |||
| EcoRI | 5′G↓AATTC | 5′AATTGGTACGCAGTCTAC | 5′GTAGACTGCGTACCAATTC | peco |
| CTTAA↓G | 3′CCATGCGTCAGATGCTC |
a
One of the oligonucleotides of each adaptor was phophorylated at its appropriate 5′ site. Adaptors were chosen from references 4, 8, and 15.