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. Author manuscript; available in PMC: 2006 Jul 11.
Published in final edited form as: Endocrinology. 2001 Mar;142(3):1167–1178. doi: 10.1210/endo.142.3.8021

FIG. 2.

FIG. 2

Dmrt1 mRNA levels change during postnatal development of the testes. RNA was isolated from testes of different aged rats, including E18 (embryonic day 18) and postnatal days 5 (P5), 10 (P10), 15 (P15), 20 (P20), 40 (P40), and 60 (P60). Fifteen micrograms of total testis RNA was assayed for both Dmrt1 and actin mRNA levels using RNase protection analysis with approximately 5 × 105 cpm of the Dmrt1 antisense probe and 1 × 104 cpm of the antisense actin probe (see Materials and Methods). Following overnight hybridization and digestion with RNase the samples were run on a 5% denaturing gel to resolve protected fragments of 151 bp (Dmrt1) and 126 bp (actin). Arrows mark the protected fragments. Transfer RNA (tRNA) is added as a negative control for the formation of protected fragments.