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. Author manuscript; available in PMC: 2006 Jul 11.
Published in final edited form as: Endocrinology. 2001 Mar;142(3):1167–1178. doi: 10.1210/endo.142.3.8021

FIG. 3.

FIG. 3

Dmrt1 mRNA is expressed in testicular Sertoli cells. Dmrt1 and actin mRNA levels were measured by RPA as described in Fig. 2. A, Sertoli cells were isolated from testes of 15 day-old rats as described in Materials and Methods and RNA was isolated either immediately following completion of the preparation (Fresh d15 SC) or after various times in culture (24, 48, or 72 h) and in cells that either did (+) or did not receive a hypotonic shock to remove contaminating germ cells. RNA isolated from myoid cells, a testicular cell type that surrounds the seminiferous epithelium, was also assayed for Dmrt1. Dmrt1 and actin mRNA levels were also measured in intact tubule fragments either immediately following isolation (fresh d15 tubulutes) or after 24 h in culture (cultures tubules 24 h). B, Dmrt1 and acting mRNAs were measured by RPA in intact rat (20 days of age) and mouse (25 days of age) testes and in two mouse Sertoli cell lines, MSC-1 and TM4. Two separate isolates of the TM4 cell line were characterized. Arrows mark the protected fragments. tRNA was added as a negative control for the formation of protected fragments.