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. Author manuscript; available in PMC: 2006 Jul 11.
Published in final edited form as: Endocrinology. 2001 Mar;142(3):1167–1178. doi: 10.1210/endo.142.3.8021

FIG. 9.

FIG. 9

Serum has little impact on Dmrt1 mRNA levels but induces c-fos expression. Analysis of Dmrt1, c-fos, and actin mRNA levels in Sertoli cells treated with FBS. Sertoli cells were cultured in absence (no treatment) or the presence of 15% FBS as described in Materials and Methods and RNA isolated from the cells at the designated times. Dmrt1 (A) and c-fos (B) mRNAs were measured together with actin mRNA by RPA as described (2). For the no treatment sample, RNA was isolated at the zero time point. C, The optical densities of the protected fragment for each RNA (A and B) was quantified from the autoradiograms and the optical densities of Dmrt1 or c-fos were normalized to that for actin. The Dmrt1/actin or c-fos/actin ratio from each treatment was made relative to the no treatment ratio for the respective mRNA and the relative densitometric units plotted in the graph.