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. 2000 Sep;11(9):2987–2998. doi: 10.1091/mbc.11.9.2987

Figure 2.

Figure 2

The U2 locus transcribes more than preU2 RNA. (A) Map of the U2 repeat indicating different probes. (B) A 4.3-kb fragment from the U2 gene locus (red) that does not contain any U2 coding sequences overlaps the focus of RNA detected with the full-length U2 locus probe. Overlap appears yellow. This indicates that DNA outside of the U2 coding sequence is transcribed. (C–F) A 1.8-kb probe detects both U2 RNA in CBs and the RNA focus seen with the full-length probe. (C) U2 RNA signals seen with a full-length 6.1-kb probe. (D) U2 RNA detection with a 1.8-kb probe containing the U2 gene. (E) CB detection. (F) Composite of all three colors. (G–J) U2 coding sequence detects only U2 in CBs with faint nucleoplasmic speckle staining. (G) U2 RNA signals seen with a full-length 6.1-kb probe. (H) U2 RNA detection with a U2 coding sequence probe. (I) CB detection. (J) Composite of all three colors.