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CBs contain preU2 RNA. (Column A) 5′ U2 oligonucleotide (red) recognizes U2 in CBs (green) and more diffuse U2 in speckles (SC-35 domains). (Column B) 3′ U2 oligonucleotide (red) also recognizes U2 in CBs (green) and more diffuse U2 in speckles. (Column C) The preU2 oligonucleotide (red) detects preU2 in CBs (green) only. (Column D) The preU2 control oligonucleotide (red) does not produce any signal above background.
