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PreU2 is found throughout the CB. To show conclusively that preU2 is inside the CB and not next to or around it, we performed a three-dimensional analysis of overlap between the preU2 signal and coilin staining. Shown are three optical sections through a large CB that are ∼0.2 μm apart. These sections indicate that the preU2 and coilin signals are completely coincident within the CB.
