TABLE 2.
Primer and adapter oligonucleotide sequences
| Primer or adapter oligonucelotide | Sequence (5′ to 3′)a |
|---|---|
| Mating type primers | |
| MFαU | TTCACTGCCATCTTCACCACC |
| MFαL | TCTAGGCGATGACACAAAGGG |
| STE20aSFU | TCCGATTGCTGCGATTTGCC |
| STE20aSFL | GCGCCTGCACCATAATTCACC |
| EcoRI adapters | |
| EA1 | CTCGTAGACTGCGTACC |
| EA2 | CATCTGACGCATGGTTAA |
| Msel adapters | |
| MA1 | GACGATGAGTCCTGAG |
| MA2 | TACTCAGGACTCAT |
| Preamplification primers | |
| EcoRI-C | GACTGCGTACCAATTCC |
| EcoRI-G | GACTGCGTACCAATTCG |
| EcoRI-T | GACTGCGTACCAATTCT |
| Msel-C | GATGAGTCCTGAGTAAC |
| Msel-G | GATGAGTCCTGAGTAAG |
| Msel-T | GATGAGTCCTGAGTAAT |
| Selective primers | |
| EcoRI-CA | 6 FAM-GACTGCGTACCAATTCCA |
| EcoRI-GT | 6 FAM-GACTGCGTACCAATTCGT |
| EcoRI-TG | 6 FAM-GACTGCGTACCAATTCTG |
| Msel-CA | GATGAGTCCTGAGTAACA |
| Msel-GA | GATGAGTCCTGAGTAAGA |
| Msel-GT | GATGAGTCCTGAGTAAGT |
| Msel-TG | GATGAGTCCTGAGTAATG |
a
Sequences are 5′ to 3′, except for those of EA2 and MA2, which are 3′ to 5′.