TABLE 3.
Comparison of conventional PCR and real-time PCR
Sample and patient no. | Conventional PCR result
|
Real-time PCR resultc
|
||
---|---|---|---|---|
Aaa | Pgb | % of Aa | % of Pg | |
Saliva | ||||
1 | − | + | NDd | 0.20 ± 0.02 |
2 | + | − | 0.19 ± 0.04 | ND |
3 | + | + | 0.22 ± 0.04 | 1.56 ± 0.06 |
4 | + | − | 0.20 ± 0.03 | 5.11 × 10−4 ± 1.16 × 10−4 |
5 | − | + | ND | 0.64 ± 0.02 |
6 | − | + | ND | 1.34 ± 0.13 |
7 | + | + | 0.11 ± 0.01 | 0.08 ± 0.00 |
8 | − | + | ND | 0.81 ± 0.06 |
9 | − | − | ND | ND |
10 | − | − | ND | ND |
Subgingival plaque | ||||
1 | − | + | ND | 1.33 ± 0.20 |
2 | − | − | 7.16 × 10−4 ± 0.47 × 10−4 | ND |
3 | − | − | 4.69 × 10−3 ± 0.45 × 10−3 | ND |
4 | + | − | 0.11 ± 0.03 | ND |
5 | − | + | ND | 0.38 ± 0.04 |
6 | − | + | ND | 6.40 ± 0.43 |
7 | + | + | 0.02 ± 0.00 | 0.20 ± 0.02 |
8 | − | + | ND | 1.10 ± 0.16 |
9 | − | − | ND | ND |
10 | − | − | ND | ND |
Aa, A. actinomycetemcomitans.
Pg, P. gingivalis.
Data are expressed as means ± standard deviation (n = 3).
ND, not detected.