TABLE 1.
Phenotypic and genotypic characterization of the 50 MRSA isolates by different methods
| Group and isolate | Antibiotic susceptibility toa:
|
Phage typing result
|
Pulso- type | Mutationc
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| E | T | C | F | R | V | Tri | G | Cip | Basic international set | Experimental sets | mecI | mec promoter | ||
| nmMRSA | ||||||||||||||
| 1 | ||||||||||||||
| J15 | S | S | S | S | S | S | S | S | S | 29/52/52A/80/3A/3C/55/6/42E/47/53/54/75/77/84/95 | 67R | A | − | |
| J17 | R | R | S | S | S | S | S | S | S | 29/52/52A/80/3A//55/6/42E/47/53/54/75/77/84/81/95 | 67R | A3 | − | 6 |
| J27 | S | S | S | S | S | S | S | S | S | 6/42E/47/53/54/83A/85/90 | 47T/56B/56C/90A/1648/13M/M5/F30/MR12/MR25 | B | − | 6 |
| J28 | S | S | S | S | S | S | S | S | S | 29/52/52A/79/80/3A/3C/55/6/42E/47/53/54/75/77/84/81/94/9 | 56A/87M/MR12 | A1 | 1 | 6 |
| J29 | S | S | S | S | S | S | S | S | S | 29/52/80/53/77/95/90 | 47T/MR12 | C | − | 6 |
| J32 | S | S | S | S | S | S | S | S | R | 6/42E/47/54/77/84/81 | MR12 | A | − | |
| J38 | S | S | S | S | S | S | S | S | S | 29/52/52A/80/3A/3C/6/42E/47/54/95 | 67R | A2 | − | 6 |
| J41 | S | S | S | S | S | S | S | S | S | 29/52/42A/80/3A/6/42E/47/53/54/77/95 | NTb | A4 | − | 7, 8 |
| J61 | R | R | S | S | S | S | R | S | S | NT | MR12/MR25 | D | 2 | |
| J70 | S | S | S | S | S | S | S | S | S | 29/52/52A/80/3A/6/42E/47/53/54/95 | NT | A1 | 2 | 6 |
| 2 | ||||||||||||||
| J40 | S | S | S | S | S | S | S | S | R | NT | NT | R1 | − | 7 |
| J75 | S | S | S | S | S | S | S | S | R | NT | NT | S | − | |
| J76 | S | S | S | S | S | S | S | S | R | NT | NT | Q | − | |
| J77 | S | S | S | S | S | S | S | S | R | NT | NT | Q | − | |
| J78 | S | S | S | S | S | S | S | S | R | NT | NT | Q1 | − | |
| J79 | S | S | S | S | S | S | S | S | R | NT | NT | Q | − | |
| J80 | S | S | S | S | S | S | S | S | R | NT | NT | Q | − | |
| J81 | S | S | S | S | S | S | S | S | R | NT | NT | Q2 | − | |
| J82 | S | S | S | S | S | S | S | S | R | NT | NT | Q | − | |
| J83 | S | S | S | S | S | S | S | S | R | NT | NT | Q | − | |
| J84 | S | S | S | S | S | S | S | S | R | NT | NT | S | − | |
| 3 | ||||||||||||||
| J33 | S | S | S | S | S | S | S | S | R | NT | F30 | R | 3 | |
| J37 | R | R | S | S | S | S | S | S | S | 75/84 | NT | E | − | 6 |
| J39 | S | R | S | R | S | S | S | S | S | NT | NT | S | − | 6 |
| J58 | R | S | R | S | S | S | S | S | R | NT | NT | Q | − | |
| J59 | R | S | R | S | S | S | S | S | R | NT | NT | Q | − | |
| J62 | R | S | R | S | S | S | S | S | S | NT | NT | Q | − | |
| J63 | R | S | R | S | S | S | S | S | S | NT | NT | R | − | |
| J64 | R | S | R | S | S | S | S | S | S | NT | NT | R | − | |
| mMRSA | ||||||||||||||
| J1 | R | R | S | S | S | S | R | R | R | 84/85/88 | 47T/56A/87M/622/F38/MR8/MR12/MR25 | G | 3 | |
| J5 | R | R | S | S | S | S | R | R | R | 85/88 | 47T/622/M5/MR8/MR25 | I | 3 | |
| J7 | R | R | S | S | S | S | R | R | R | 85/88 | 47T/622/M5/F38/MR8/MR25 | G1 | 3 | 9 |
| J12 | R | S | S | S | S | S | R | R | R | 84/85/88 | 47T/87M/622/M5/F38/MR8/MR12/MR25 | H | − | |
| J13 | R | R | S | S | S | S | R | R | R | 6/53/54/75/77/83A/84/85/90/88 | 47T/56A/56B/56C/1648/67R/87M/622/M3/M5/F33/F38/MR8/MR12/MR25 | G2 | 3, 4 | |
| J18 | R | R | S | S | S | S | R | R | R | 29/6/53/54/75/83A/84/85/88 | 47T/56A/56B/56C/1648/67R/87M/13M/622/M3/M5/F33/F38/MR8/MR12/MR2 | G2 | 3 | |
| J19 | R | R | S | S | S | S | R | R | R | 29/85/88 | 47T/622/M3/M5/F33/F38/MR8/MR12/MR25 | H1 | 3, 4 | |
| J20 | R | R | S | S | S | S | R | R | R | 83A/85/88 | 47T/87M/M5/F38/MR12/MR25 | H1 | 3 | |
| J21 | R | R | S | S | S | S | R | R | R | 85/88 | 47T/56A/622/M5/MR8/MR25 | T | 3 | |
| J23 | R | R | S | S | S | S | R | R | S | 85/88 | 47T/56A/622/M5/MR8/MR25 | F | 3 | |
| J24 | R | R | S | S | S | S | R | R | R | 83A/85/88 | 56A/56B/56C/67R/87M/622/F30/F33/F38/MR8/MR12/MR25 | F1 | 3, 4 | |
| J25 | R | R | S | S | S | S | R | R | R | 83A/85/88 | 56A/56B/56C/67R/87M/622/M3/MR8/MR12/MR25 | J | 3 | |
| J26 | R | R | S | S | S | S | R | R | R | NT | NT | K | 5 | |
| J85 | R | R | S | S | S | S | R | R | R | 84/85/88 | 47T/56A/87M/622/M3/M5/F30/F33/F38/MR8/MR12/MR25 | H2 | 3 | |
| Archaic | ||||||||||||||
| J42 | R | R | R | S | S | S | S | S | S | 6/42E/47/53/54/77/84/85 | 622/M5/F33/F38/MR25 | M | − | |
| J44 | R | R | S | S | S | S | S | S | S | 79/6/42E/47/53/54/75/77/83A//84/85/81/90 | 47T/56A/1648/67R/87M/622/M5/F30/F33/F38/MR25 | O | − | |
| J49 | S | R | S | S | S | S | S | S | S | 47/53/75/77/83A/84/85/90 | 47T/56A/67R/87M/F38 | O1 | − | |
| J50 | R | R | S | S | S | S | S | S | S | 75/77/84 | NT | O1 | − | |
| J51 | R | R | S | S | S | S | S | S | S | 6/42E/47/53/54/77/84/85 | 622/M5/F33/F38/MR25 | N | − | |
| J53 | R | R | S | S | S | S | S | S | S | 6/42E/47/53/54/77/84/85 | 622/M5/F33/F38/MR25 | P | − | |
| J54 | R | R | S | S | S | S | S | S | S | 79/6/42E/47/53/54/75/77/83A//84/85/81/90 | 47T/56A/1648/67R/87M/622/M5/F30/F33/F38/MR25 | O3 | − | |
a
E, erythromycin, T, tetracycline; C, chloramphenicol; E, fusidic acid; R, rifampin; V, vancomycin; Tri, trimethoprim; G, gentamicin; Cip, ciprofloxacin; R, resistant; S, susceptible. All 50 strains were resistant to penicillin and methicillin and sensitive to rifampin and vancomycin.
b
NT, nontypeable.
c
−, mec1 deletion.
d
I, 202C→T; 2, 324T→G; 3, 93A→G; 4, 5-G insertion; 5, 208A→T; 6, G→T mecA SD; 7, A insertion upstream of mecA SD; 8, A deletion at mecA −35; 9, G insertion in mecA SD.