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Identification of protein bands for mass spectrometry. (A) Silver-stained 4 to 12% acrylamide gel with a lysate of B. microti isolated from infected RBCs. (B) Immunoblot of an identical gel that was probed with sera from B. microti-infected patients. The band of interest is denoted with an arrow; both variants of BMN1-21 [BM11-5(a) and BM11-51(b)] were isolated from this band. MW, molecular weight, in thousands.
