Figure 2.

Detection of Cα1 and C_s mRNA in various species. RT-PCR of total RNA from ovine and murine testes and PCR of cDNA from human testis. The forward primers used to generate the PCR products are shown at the top of the lanes: oC_s(−11) to amplify C_s and Cαa to amplify Cα1. The reverse primer in all cases was CαeR. The PCR products were subjected to electrophoresis in an 0.8% agarose gel and stained with ethidium bromide. Cα1 and C_s products were obtained from all three species. Controls in which the RT was omitted yielded no bands. Lane M, DNA molecular mass markers (in kilobases).